Figure 2.

The effects of loss of function of Moe1 on MT assembly and stability. (A) The percentages of cells with either a long MT (one that spans at least half the cell length) or an intact spindle (one that connects the two nuclei) are plotted against time after the cold shock. (B) Cells first were cold-shocked in ice for 30 min. The MTs then were examined at various time points after recovery at 20°C. (C) α1 tubulin mutant (nda2^cs) and the α1-tubulin Moe1Δ double mutant cells (nda2^cs moe1Δ) pregrown at 30°C to log phase were shifted to 20°C; the MTs were visualized after 30 min. (D) Serial dilutions of cells (1:5) were spotted on yeast extract medium supplemented with adenine and uracile (YEAU) plates (Materials and Methods) containing either no or 12.5 μg/ml of thiabendazole. The plates were incubated at 30°C for 3 days. Note that the plating efficiency of moe1Δ cells at 30/25°C is only 50% of wild type. We therefore routinely use twice as many moe1Δ as wild-type cells for this type of analysis. The wild-type and moe1Δ strains in A and D were SP870 and MOE1U, respectively, and in B were PN1 and PNMOE1L, respectively.