1 |
Technique |
AFLP |
Full abbreviation of type of DNA markers, such as AFLP, SSR, RAPD |
2 |
Experiment |
EcoRI_ACA-MseI_CAC |
For VNTR, RAPD and SSR markers, primer sequences will be given in full. In this example, a six-nucleotide cutting enzyme EcoRI and a four-nucleotide cutting enzyme MseI were used to digest genomic DNA. PCR reaction was conducted with an EcoRI primer with selective nucleotides ACA, and a MseI primer with selective nucleotides CAC |
3 |
Source |
D Sonia id0011 |
Biological source of DNA. The binomial system with abbreviation of genus followed by species name is used to designate biological species. It is followed by a four-digit ID number to identify individual samples. In this example, DNA was isolated from a plant belonging to genus Dendrobium, species Sonia, with a lab-assigned ID 0011 |
4 |
UnitSize |
1 |
Unit size of DNA fragment to define the unit spacing. It can also be interpreted as the sequence resolution. In this example, the 1 indicates that the sequence spacing is 1 base pair. |
5 |
StartSize |
50 |
Size of the first DNA fragment (in base pairs) |
6 |
EndSize |
90 |
Size of the last DNA fragment. This field is redundant, and is kept mainly for proof-reading |
7 |
Annotation |
AFLP profile for D. Sonia |
Annotation of the fingerprint (only if required) |
8 |
Signature |
A..B..DC..B.D.....CC...A.....DDA.A....C..D..BB |
Alternatively, a system employing the symbols [4,3,2,1] for peaks and [0] for peak-absence may also be used. |