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. 2003 Mar;23(5):1674–1687. doi: 10.1128/MCB.23.5.1674-1687.2003

FIG. 7.

FIG. 7.

LXXLL motifs in armadillo repeats of β-catenin are not required for the interaction with androgen receptor LBD. (A) CV-1 cells in a mammalian two-hybrid assay were cotransfected with 10 ng of GAL4/AR LBD, 100 ng of pFR-LUC, 100 ng of wild-type β-catenin, individual LXXLL→AXXAA mutants, or an equimolar amount of pcDNA3.1 (B) CV-1 cells in a transactivation assay were cotransfected as described for panel A except that pCMVhAR507-919 and MMTV-LUC were used. (C) Western blot analysis of wild-type β-catenin and its mutants in SKBR3 cells, chosen for this analysis because they have very low endogenous expression of β-catenin.