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Expression and tyrosine phosphorylation of mutant Tor proteins. Lysates were prepared from embryos expressing various Tor mutant proteins. Tor proteins were immunoprecipitated, and the precipitates were examined by immunoblot analysis using antibodies recognizing Tor (αTor). Blots then were stripped and reprobed with antibodies recognizing phosphotyrosine (αP-Y). The position of Tor and P-Y Tor is indicated on the right by an arrow.
