FIG. 1.

Structure and expression of chimeric proteins. (A) Schematic representation of the encoded chimeric fusion proteins. Abbreviations: PAK, PAK domain; PH, pleckstrin homology domain; int, protein-protein interaction domain. (B) Each chimera contains a C-terminal triple HA epitope fusion that was utilized to assess protein expression as described in Materials and Methods. Pgk1 served as a loading control. The band found above all samples in the Cla4-containing blot (probed with monoclonal HA antibody) is a background band commonly observed when using this antibody.