TABLE 1.
Yeast strains
| Strain | Genotype | Source |
|---|---|---|
| SY3357a | MATaleu2-Δ1 ura3-52 his3-Δ200 trp1-Δ63 ade8Δ ade2-101 mfa2-Δ1::FUS1-lacZ | D. Mitchell and G. Sprague |
| SY3360a | MATaleu2-Δ1 ura3-52 his3-Δ200 trp1-Δ63 ade8Δ ade2-101 mfa2-Δ1::FUS1-lacZ cla4::TRP1 | D. Mitchell and G. Sprague |
| SY3748a | MATα leu2-Δ1 ura3-52 his3-Δ200 trp1-Δ63 ade8Δ ade2-101 mfa2-Δ1::FUS1-lacZ cla4::TRP1 ste20::HIS3 〈pRS316ADE8CLA4〉 | This study |
| SY3749a | MATaleu2-Δ1 ura3-52 his3-Δ200 trp1-Δ63 ade8Δ ade2-101 mfa2-Δ1::FUS1-lacZ ste20::HIS3 | This study |
| SY3750a | MATaleu2-Δ1 ura3-52 his3-Δ200 trp1-Δ63 ade8Δ ade2-101 mfa2-Δ1::FUS1-lacZ ste20::HIS3 bar1::kanamycin | This study |
| SY3751a | MATaleu2-Δ1 ura3-52 his3-Δ200 trp1-Δ63 ade8Δ ade2-101 mfa2-Δ1::FUS1-lacZ cla4::TRP1 cln1::HIS3 cln2::HIS3 〈pRS316ADE8CLA4〉 | This study |
| SY3752b | MATα Σ ura3-52 ste20::ura3− (made ura3− on 5-FOA plate) leu2::URA3 | April Goehring |
| SY3753a | MATaleu2-Δ1 ura3-52 his3-Δ200 trp1-Δ63 ade8Δ ade2-101 mfa2-Δ1::FUS1-lacZ ste20::HIS3 ssk1::URA3 | This study |
| SY3970a | MATa/α leu2-Δ1 ura3-52 his3-Δ200 trp1-Δ63 ade8Δ ade2-101 mfa2-Δ1::FUS1-lacZ ste20::HIS3 | April Goehring |
| SY3971a | MATα leu2-Δ1 ura3-52 his3-Δ200 trp1-Δ63 ade8Δ ade2-101 mfa2-Δ1::FUS1-lacZ ste20::TRP1 | David Mitchell |
| 227 | MATα lys1 cry1 | Ira Herskowitz |
a
These yeast strains are derivatives of YPH499 and YPH500 (S288C) (39).
b
This yeast strain is a derivative of HY334 (of the Σ1278 background) provided by G. Fink, Whitehead Institute for Biomedical Research, Cambridge, Mass.