TABLE 2.

Abilities of chimeric constructs to function in the pheromone response pathway^a

^aThe data show the abilities of chimeric fusions to restore pheromone response to ste20Δ strains. Cells having STE20 deleted (SY3749) were transformed with pRS315-derived plasmids carrying full-length STE20, full-length CLA4, or a chimeric gene fusion. The resultant strains were tested for the ability to express FUS1-lacZ (β-galactosidase activity), the ability to form mating projections, and the ability to mate as described in Materials and Methods. ND, not determined. All assays were done in triplicate. Standard deviations were 10% or less.