FIG. 5.

Functional genomic strategy to identify Kap142p as a nuclear transport factor. The kap142 mutation allows VDE to enter the nucleus, implying that Kap142p is a nuclear transport factor for VDE; none of the other six karyopherin β mutants exhibited VDE localization in the nucleus except when treated with rapamycin. (A) Effects of several karyopherin mutations on cellular distribution of VDE. Exponentially growing cells of each mutant were either left untreated or treated with 0.2 μg of rapamycin per ml for an hour. The localization of VDE was examined by indirect immunofluorescence after staining with the anti-VDE antibody. Yeast nuclei were stained with DAPI. (B) The numbers of cells with nuclear fluorescence were counted, and the fractions were calculated as in Fig. 4B.