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. 2003 Mar;23(5):1726–1736. doi: 10.1128/MCB.23.5.1726-1736.2003

FIG. 8.

FIG. 8.

Artificial mislocalization of VDE causes homing even in mitotically growing cells. (A) Detection of homing products in rapamycin-treated mitotic cells. S. cerevisiae genomic DNA was prepared from a log-phase culture of YOC2758 without rapamycin treatment (left) or with rapamycin treatment for an hour (right) and subjected to semiquantitative PCR analysis. The upper and lower panels show amplified DNA derived from the homing product and the amplified RHO1, respectively, as in Fig. 1C. (B) Immunolocalization of NLS-tagged VDE in mitosis. YOC2758 and YOC2816 (expressing NLS-VDE) were grown to early log phase in YPD. The localization of VDE was examined by indirect immunofluorescence staining with the anti-VDE antibody. S. cerevisiae nuclei were stained with DAPI. (C) Effects of NLS on homing frequency. S. cerevisiae genomic DNA was prepared from log-phase cultures of YOC2758 (left) and YOC2816 (right) and subjected to semiquantitative PCR analysis. Data are presented as in Fig. 1C.