FIG. 3.

Myr-Aurora-A did not activate Ras in Xenopus oocytes. Oocytes were injected with Raichu-Ras DNA (directly into the germinal vesicle) and incubated for 36 h. Oocytes showing clear fluorescence were selected and divided into four groups, each of which received a second injection of water, RasGRF/CDC25 (germinal vesicle injection with DNA), Myr-Aurora-A (20 ng of mRNA per oocyte), or Myr-Aurora-A-KA (20 ng of mRNA per oocyte). Following overnight incubation, FRET ratios (527/475 nm) were measured and expressed as a percentage (means with standard deviations of three independent experiments) of the control (Raichu-Ras + water).