FIG. 5.

Myr-Aurora-A-injected oocytes contained sustained MPF activity following GVBD. (A) Oocytes were either left unstimulated (germinal vesicle [GV]) or stimulated with progesterone. Individual oocytes were lysed at the time of appearance of the maturation spot (GVBD) or at the indicated time following GVBD. Extracts were subjected to MPF assays (upper panel) or immunoblotting with antibodies against cyclin B2 (middle panel) or MAP kinase (lower panel). Shown is a representative of three independent experiments. xMAPK, Xenopus MAP kinase. (B) Oocytes were injected with water (germinal vesicle) or with Myr-Aurora-A mRNA. Individual oocytes were lysed and analyzed as in A. Shown is a representative of three independent experiments. (C) MPF activity was monitored in oocytes injected with Myr-Aurora-A mRNA exactly as described for B. (D) Oocytes were injected with water (germinal vesicle) or v-ras mRNA (36) (5 ng/oocyte). Individual oocytes were lysed at GVBD or the indicated time following GVBD. Extracts were analyzed for MPF activities (upper panel) or Xenopus MAP kinase phosphorylation (lower panel).