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War1p localizes to the nucleus and is able to form homodimers. (A) YAK122 cells expressing a War1p-GFP fusion were grown in YPD to the exponential growth phase and stressed with 8 mM sorbate for 2 h (d to f) or left untreated (a to c). Living cells were treated with DAPI for 15 min to stain nuclear DNA and inspected in a Zeiss Axiovert 10 fluorescence microscope. (a and d) Fluorescein isothiocyanate filter, War1-GFP; (b and e) DAPI staining; (c and f) Nomarski image.
