FIG. 4.

War1p recognizes a cis-acting WARE in the PDR12 promoter. (A) FY1679-28c transformed with plasmids carrying the indicated fragments of the PDR12 promoter fused to lacZ were grown to the logarithmic growth phase, shifted to pH 4.5 YPD, and treated with 1 mM sorbate. β-Galactosidase assays were performed in triplicate in the absence (−) and presence (+) of sorbate, and activity is given in Miller units. (B) FY1679-28c (wild type [WT]) and YAK100 (Δwar1) cells transformed with pRW95-3 and pRW95-W1 were grown in SC medium to the logarithmic growth phase, shifted to pH 6.8 YPD, and stressed with 8 mM sorbate. β-Galactosidase activity was assayed, and activities are given in Miller units. Three independent experiments were performed with each construct, and standard deviations are given in parentheses.