FIG. 8.
Western blot analysis of protein fractions from kidney and mammary tissues of floxed Trsp and promoter-specific transgenes. Protein samples were separated from Trspfl/fl (control), Trspfl/fl-WAP-Cre (WAP-Cre), and Trspfl/fl-MMTV-Cre (MMTV-Cre) mice by SDS-PAGE and blotted with antibodies against Brca1, p53, and actin (as control), and band intensities were quantitated as described in Materials and Methods. Representative Western blots are given in the figure from triplicate experiments with tissues from two different sets of animals. Brca1 was reduced by 67% ± 8%, and p53 was increased by 400% ± 20% (mean values ± standard deviation).