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. 2003 Mar;23(5):1750–1763. doi: 10.1128/MCB.23.5.1750-1763.2003

FIG. 8.

FIG. 8.

Pheromone response in single and multiple mutants of far3 and far7 to far11. (A) Growth on pheromone. Serial dilutions of wild-type (WT) and mutant strains were spotted onto YEPD alone and YEPD + 0.1 μg of α-factor per ml. Growth was assessed after 3 days at 30°C. The experiment was repeated more than 20 times with the same results. The results of a typical experiment are shown. (B) Signal transduction. Wild-type and mutant strains containing a genomically integrated FUS1::lacZ reporter were tested for basal (no α-factor) and induced (plus α-factor) levels of β-galactosidase, as described in Materials and Methods. Each time the assay was performed, four independent isolates of each strain were grown to log phase and then either assayed directly or induced with α-factor before the β-galactosidase levels were measured. The assay was repeated three times, with similar results. The results of a typical experiment is shown. For all experiments, strains were as follows: WT, SY2227; ste12, SY4085; far1, SY2673; far3, SY4062; far7, SY4059; far8, SY4061; far9, SY4060; far10, SY4063; far11, SY4064; sextuple far3 far7 to far11, SY4075.