TABLE 2.
Plasmids used in this study
Name | Description | Source or reference |
---|---|---|
pGBDU-C1 | Two-hybrid Gal4BD plasmid | 33 |
pGAD-C1 | Two-hybrid Gal4AD plasmid | 33 |
pGAD-C3 | Two-hybrid Gal4AD plasmid | 33 |
pKEB35 | HA epitope with HpaI sites | 3 |
pOBDCYH | Two-hybrid Gal4BD plasmid | 6 |
pSL2252 | Single-copy Far3 | 30 |
pSL2310 | far3::LEU2 replacement plasmid | 30 |
pSL2367 | Single-copy Far3 with C-terminal HpaI sites | Horecka, un- published |
pSL2771 | HA epitope expression plasmid | 34 |
pSL2783 | Single-copy Far3-HA | This study |
pSL2784 | High-copy-number Far3-HA | This study |
pSL2785 | Two-hybrid Gal4BD-Far3 fusion | This study |
pSL2786 | Two-hybrid Gal4BD-Far7 fusion | This study |
pSL2787 | Two-hybrid Gal4BD-Far9 fusion | This study |
pSL2788 | Two-hybrid Gal4BD-Far3 fusion | This study |
pSL2789 | Two-hybrid Gal4AD-Far7A fusion | This study |
pSL2790 | Two-hybrid Gal4BD-Far8 fusion | This study |
pSL2791 | Two-hybrid Gal4BD-Far9A fusion (larger) | This study |
pSL2792 | Two-hybrid Gal4BD-Far9B fusion (smaller) | This study |
pSUL-16 | ste12::LEU2 replacement plasmid | 19 |