FIG. 2.

Multiple sequence alignment of Nob1p and related proteins. The full sequences of Nob1p and its putative eukaryotic orthologues were aligned using T-Coffee and corrected manually where required. Sequences are identified by the SwissProt number and species abbreviation. Numbers within the sequences indicate residues omitted due to unreliable alignments in these regions. Letters and symbols on the consensus line are as follows: s, small residues; t, tiny; b, big; h, hydrophobic; a, aromatic; l, aliphatic; p, polar; c, charged; −, negatively charged; +, positively charged. Individual residues with more than 80% identity in the entire alignment are shown as capital letters on the consensus line. Conserved acidic (Asp/Glu) residues in the PIN domain are denoted by red asterisks above the alignment. Conserved cysteines marked by blue asterisks are part of the zinc ribbon motif. The figure was prepared using CHROMA (10).