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Depletion of Nob1p inhibits growth. (A) Growth rates of the GAL::HA-nob1 (squares) and wild-type (diamonds) strains following a shift from galactose to glucose medium. (B) Western blot analysis of Nob1p depletion. Whole-cell extracts were prepared from samples harvested at the indicated times. Equal amount of proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (10% polyacrylamide), and the HA tag on HA-Nob1p was detected by Western blotting.
