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. 2003 Mar;23(5):1647–1655. doi: 10.1128/MCB.23.5.1647-1655.2003

FIG. 6.

FIG. 6.

Impairment of pH-dependent processing of PacC in mutants lacking the YPXL/I motifs. The wild type and the indicated mutant strains were grown under acidic conditions (H+) and shifted for 45 min to alkaline conditions (OH−). Protein extracts were analyzed by EMSA (A) and Western blot analysis with anti-PacC(5-205) antiserum (B). The positions of the different PacC polypeptides (B) or of the corresponding complexes with DNA (A) are indicated by arrows. Protein markers are shown in kilodaltons.