FIG. 4.
LexA coactivates gene expression in yeast. (A) Shown is an S1 assay for the inducible LexA strain grown in glucose (Glu) or galactose (Gal) with 0 or 10 mM aminotriazole (AT). The control sample is a parallel reaction lacking RNA. Total HIS3 mRNA levels from +1 and +13 initiation sites are quantified and normalized to DED1 levels. (B) The average HIS3 mRNA levels and standard errors from quantitative S1 assays are presented normalized to the glucose without aminotriazole condition for the following strains: LexA−, inducible LexA, mtGRE, mtLexAop, and 154-bp spacer. (C) Flow cytometric analysis was performed on GFP reporter strains grown in galactose. The histogram depicts the number of cells and their fluorescence intensity (log scale) for a representative run. The medians for LexA− and inducible LexA strains are 4.7 and 8.2 fluorescence units.