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Peroxidase activity of wild-type and mutant KatGsa
| KatG | kcat (s−1) | Km (μM) | kcat/Km (M−1 s−1) | kcat/Km ratio, mutant/wild type |
|---|---|---|---|---|
| Wild type | 3.48 ± 0.13 | 84.2 ± 7.3 | 4.13 × 104 | 1.00 |
| A110V | 4.85 ± 0.24 | 64.3 ± 1.7 | 7.54 × 104 | 1.83 |
| A139P | 1.69 ± 0.15 | 95.7 ± 18.6 | 1.77 × 104 | 0.43 |
| S315N | 0.86 ± 0.09 | 80.6 ± 14.1 | 1.07 × 104 | 0.26 |
| L619P | 1.06 ± 0.06 | 78.2 ± 9.5 | 1.36 × 104 | 0.33 |
| L634F | 0.99 ± 0.03 | 65.4 ± 5.0 | 1.51 × 104 | 0.37 |
| D735A | 0.99 ± 0.08 | 62.9 ± 10.2 | 1.57 × 104 | 0.38 |
See Table 2, footnote a.
