TABLE 3.

Characteristics of 27 ESBL-producing strains and 11 transconjugants

Isolate no.a	Wardb	Source	Organismc	MIC (μg/ml)d					Zone diame of FOX (mm)	pI(s) of β-lactamase(s)	β-Lactamase(s)	Plasmid(s) (kb)
				CTX	CTX/cla	CAZ	CAZ/cla	IMP
CH1	MW1	Sputum	eco	64	1	16	8	1	6	5.4, 7.6, 8.3	TEM-1, SHV-1, SHV-like	47, 7, 5, 2
CH1T	MW1	Sputum	eco	64	0.5	4	0.5	0.25	30	5.4, 7.6, 8.3	TEM-1, SHV-1, SHV-like	47
CH2	SW1	Urine	eco	>256	0.50	16	2	1	18	5.4, 8.6	TEM-1, CTX-M-3	42, 20, 2
CH2T	SW1	Urine	eco	>256	0.25	16	2	0.25	24	5.4, 8.6	TEM-1, CTX-M-3	42, 2
CH3	SW1	Pelvic drainage	eco	16	0.13	4	0.5	0.25	23	5.4, 8.6	TEM-1, CTX-M-3	37, 31
CH4	MW2	Urine	eco	>256	0.25	1	0.5	0.25	19	5.4, 8.0	TEM-1, SHV-43	42, 20, 1
CH5	ICU	Ascites	eco	>256	0.13	2	0.25	0.5	23	5.4, 8.6	TEM-1, CTX-M-3	42, 22
CH6	PW	Blood	kpn	>256	0.06	2	0.5	0.25	27	8.0	SHV-43	32
CH7	MW2	Blood	kpn	>256	0.06	1	0.25	0.25	28	5.4, 8.6	TEM-1, CTX-M-3	37
CH8	PW	Catheter blood	kpn	>256	0.06	4	0.25	0.25	27	5.4, 5.9, 7.6, 8.6	TEM-1, TEM-like, SHV-1, CTX-M-3	39
CH8T	PW	Catheter blood	kpn	256	0.25	4	0.5	0.25	23	5.4, 5.9, 7.6, 8.6	TEM-1, TEM-like, SHV-1, CTX-M-3	39
CH9	GW	Genital	kpn	64	0.02	1	0.25	0.5	25	5.4, 8.6	CTX-M-3	31, 9, 4, 3
CH10	SW2	Sputum	kpn	64	0.06	1	0.25	0.5	25	7.6, 8.3	SHV-1, SHV-like	39, 13
CH10T	SW2	Sputum	kpn	128	0.25	2	0.75	0.25	23	7.6, 8.3	SHV-1, SHV-like	39, 13
CH11	MW3	Sputum	kpn	>256	0.03	1	0.25	0.5	25	8.6	CTX-M-3	37
CH12	SW2	Sputum	kpn	>256	0.06	4	0.5	0.5	26	7.6, 8.3	SHV-1, SHV-like	43
CH13	MW4	Sputum	kpn	32	0.06	2	0.25	0.5	26	8.6	CTX-M-3	30
CH13T	MW4	Sputum	kpn	64	0.13	2	0.25	0.25	24	8.6	CTX-M-3	30
CH14	MW5	Sputum	kpn	32	0.06	2	0.25	0.25	22	5.4, 8.6	TEM-1, CTX-M-3	32, 4, 3, 1
CH15	ICU	Sputum	kpn	16	0.13	4	1	0.5	23	5.4, 8.6	TEM-1, CTX-M-3	37, 31, 3
CH15T	ICU	Sputum	kpn	16	0.13	2	1	0.25	23	5.4, 8.6	TEM-1, CTX-M-3	37
CH16	ICU	Pelvic drainage	kpn	>256	0.03	1	0.5	0.5	25	5.4, 8.6	TEM-1, CTX-M-3	43, 28, 4, 3, 1
CH17	PW	Catheter tip	kpn	>256	0.06	2	0.25	0.5	26	5.4, 8.6	TEM-1, CTX-M-3	37, 34, 2
CH17T	PW	Catheter tip	kpn	>256	0.13	2	0.25	0.25	23	5.4, 8.6	TEM-1, CTX-M-3	37
CH18	ICU	Abdominal drainage	kpn	>256	0.06	1	0.25	0.25	26	5.4, 8.6	TEM-1, CTX-M-3	31, 9, 4, 3
CH19	PW	Intubation	kpn	>256	0.06	4	0.5	0.5	26	8.0	SHV-43	32
CH20	MW5	Ascites	kpn	>256	0.06	1	0.25	0.5	26	8.0	SHV-43	32
CH21	PW	Umbilical swab	kpn	16	0.06	2	0.5	0.5	26	8.0	SHV-43	48, 32
CH21T	PW	Umbilical swab	kpn	4	0.13	1	0.5	0.25	29	8.0	SHV-43	32
CH22	PW	Wound	kpn	>256	0.06	2	0.25	0.5	26	5.4, 8.6	TEM-1, CTX-M-3	39
CH23	SW2	Sputum	ecl	>256	32	>256	16	32	6	5.4, 7.9, 8.6	TEM-1, AmpC-like, CTX-M-3	37, 2, 1
CH23T	SW2	Sputum	ecl	>256	0.25	32	2	0.5	23	5.4, 8.6	TEM-1, CTX-M-3	37, 1
CH24	SW3	Abdominal drainage	ecl	128	16	4	4	4	6	5.4, 8.6	TEM-1, CTX-M-3	37, 3
CH25	SW1	Sputum	ecl	>256	1	>256	8	0.5	6	5.4, 8.6	TEM-1, CTX-M-3	38, 12, 8, 3, 1
CH25T	SW1	Sputum	ecl	64	0.13	32	1	0.25	22	5.4, 8.6	TEM-1, CTX-M-3	38, 3, 1
CH26	MW3	Sputum	cfr	>256	0.25	32	2	0.5	15	5.4, 8.6	TEM-1, CTX-M-3	33, 15, 2, 1
CH26T	MW3	Sputum	cfr	64	0.13	4	1	0.25	25	5.4, 8.6	TEM-1, CTX-M-3	33
CH27	MW6	Throat swab	cfr	>256	0.13	>256	0.5	0.5	28	8.6	CTX-M-3	32
EC C600			eco	0.125	0.13	0.5	0.5	0.25	23

^aEC C600, recipient; T, transconjugant.

^bMW, medical ward; SW, surgical ward; PW, pediatric ward; ICU, intensive care unit.

^ceco, E. coli; kpn, K. pneumoniae; ecl, E. cloacae; cfr, C. freundii.

^dCTX, cefotaxime; CTX/cla, cefotaxime-clavulanic acid; CAZ, ceftazidime; CAZ/cla, ceftazidime-clavulanic acid; IMP, imipenem.

^eFOX, cefoxitin. NCCLS breakpoints: resistant, ≤14 mm; susceptible, ≥18 mm.