TABLE 2.
Resistance profiles of AAC(6′)-Ib7, AAC(6′)-Ib8, and AAC(6′)-Ib11 and correlation between resistance phenotypes and amino acids at positions 118 and 119a
| Strain (plasmid) | Amino acid at position:
|
MIC (μg/ml)
|
|||||
|---|---|---|---|---|---|---|---|
| 118 | 119 | Gen | Ami | Net | Tob | Ise | |
| E. coli | |||||||
| DH5α | 0.125 | 0.25 | 0.125 | 0.125 | 0.125 | ||
| BM694(pAZ505) | Q | L | 0.5 | 16 | 32 | 128 | 4 |
| DH5α(pLMM563) | Q | S | 4 | 2 | 8 | 8 | 0.5 |
| DH5α(pGEMM563) | Q | S | 4 | 2 | 8 | 8 | 0.5 |
| DH5α(pGEMM563 M) | L | S | 16 | 16 | 32 | 32 | 2 |
| DH5α(pLMM564) | Q | S | 2 | 1 | 4 | 8 | 0.25 |
| DH5α(pGEMM564) | Q | S | 2 | 2 | 8 | 8 | 0.25 |
| DH5α(pGEMM564 M) | L | S | 4 | 8 | 16 | 16 | 0.5 |
| DH5α(pSTI1) | L | S | 8 | 16 | 32 | 16 | 2 |
| DH5α(pGEMMST) | L | S | 8 | 16 | 32 | 16 | 2 |
| DH5α(pGEMMSTM) | Q | S | 4 | 4 | 8 | 16 | 0.5 |
| Salmonella serovar Typhimurium (pSTI1) | L | S | 16 | 32 | 64 | 32 | 4 |
a
Aminoglycoside abbreviations are as given in Table 1, footnote a. Ise, isepamicin; Kan, kanamycin.