Figure 3.

Expression of MyoR transcripts during differentiation of C2 cells. Total RNA was isolated from C2 cells maintained at subconfluent or confluent densities in growth medium or after transfer to differentiation medium for 3, 5, and 7 days. Transcripts for MyoR, MEF2C, and L7 were measured by semiquantitative reverse transcriptase–PCR. MyoR and L7 yield single PCR products of the predicted size, and MEF2C yields two products reflecting alternative splicing. In the absence of reverse transcriptase (-RT), no products were observed for L7 (Bottom), MyoR, or MEF2C (not shown).