FIG. 5.

Effects of increasing concentrations of RT inhibitors and indinavir on viral vector titer. (A) The indicated concentrations of RT inhibitors (zidovudine, didanosine, lamivudine, and stavudine) were added at the time of infection with 8 μg of Polybrene per ml and maintained through the initiation of puromycin selection. AZT, zidovudine; ddI, didanosine; 3TC, lamivudine; d4T, stavudine. (B) Indinavir was added at the time of induction with ponasterone A and maintained through the harvesting of viral supernatant for target cell transduction. Any indinavir remaining from the induction phase would be diluted 10- to 1,000-fold during the serial dilutions to calculate titer. For both panels, the results shown are the means (± standard deviations) of quadruplicate determinations and are representative of three independent experiments.