FIG. 4.

Combinatorial effects of 3TC and other HIV inhibitors in PBMCs. The same procedures were used as in Fig. 3, but two other HIV inhibitors were also included. Briefly, after adsorption at 37°C, activated PBMCs were resuspended in fresh medium and adjusted to the proper concentrations, and 10⁶ cells per well were distributed into 96-well plates containing constant concentrations of two HIV inhibitors, indinavir (INV) and nevirapine (NVP), as well as either no additional drug or various concentrations of 3TC. Half of the culture fluids were replaced at 3-day intervals. Culture fluids were tested for reverse transcriptase (RT) activity at 7 and 10 days after infection. Results shown are means of three independent measurements ± standard deviation.