TABLE 3.
IC50 values for 3TC triphosphate of wild-type reverse transcriptase and those derived from clinical and cloned samplesa
| Origin of enzyme | Mean IC50 for 3TC (μM) ± SD | Drug resistance-associated mutations | Polymorphisms (other changes) |
|---|---|---|---|
| Wild type-HxB2D | 1.59 ± 0.23 | ||
| M184V-HxB2D | 220.00 ± 14.0 | M184V | |
| RT-1 | 152.00 ± 9.60 | M41L, T69N, K70R, L100I, K103N, M184V, K219Q | K43Q, V60I, V118I, I135V, Q207E, K223Q, L228H |
| RT-2 | 4.48 ± 0.34 | E122K, D123E, I178M, G196E, Q207L, R211K, L214F | |
| RT-3 | 167.00 ± 8.70 | M41L, D67N, K70R, M184V, H208Y, T215F, K219Q | K43M, D121H, I135T, K154X, P157R, G196E, Q207H, R211K, L214F, D218E |
| RT-4 | 5.82 ± 0.36 | I94S, E122K, S134G, V245E |
a
RT-1 to RT-4 were cloned from clinical samples. The IC50 values shown are means ± standard deviation calculated from two independently performed experiments.