FIG. 1.

Measurement of the expression levels of the cyp51 and mdr genes in A. fumigatus mutants. For each target gene the expression levels were quantified by real-time PCR and then compared to the expression level of the same gene in the azole-susceptible parental strain, ND158. Expression levels of a control gene, tef3, were also determined. Differences in expression levels between the two strains are plotted for cyp51A and cyp51B (A), mdr1 and mdr2 (B), and tef3 (C). The data presented are the averages of two independent determinations. Deviations from the mean were less than 10%, and therefore error bars are not shown.