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. 1999 Jan 19;96(2):592–597. doi: 10.1073/pnas.96.2.592

Figure 2.

Figure 2

Assessment of the HAC in clone 64b5 with FISH combined with immunofluorescence to demonstrate colocalization of the L2H2UN1 probe (red, rhodamine filter) and the antibody to the active kinetochore protein, centromeric binding protein E (CENP-E; green, fluorescein isothiocyanate filter) on the HAC. Chromosomes are counterstained with 4′,6-diamidino-2-phenylindole. (Left) Rhodamine and fluorescein isothiocyanate filters merged. (Upper Right) Rhodamine filter alone. (Lower Right) Fluorescein isothiocyanate filter alone. Arrow denotes the HAC.