Skip to main content
. 1999 Jan 19;96(2):592–597. doi: 10.1073/pnas.96.2.592

Figure 3.

Figure 3

HAC microdissection products used to assess the content of the HAC in 64b5. (A) HACs were microdissected from clone 64b5 after propagation in the absence of G418 selection for 78 generations. The microdissection probe was hybridized to metaphase spreads from normal human peripheral blood cells under high-stringency conditions. (B) The same microdissection probe as in A, hybridized under identical conditions to metaphase spreads from clone 64b5, gives very strong signals on the HAC. (C) HACs were microdissected from clone 64b5 after propagation in the absence of G418 selection for 72 generations. The microdissection probe was hybridized to metaphase spreads from HT1080 cells under normal stringency and blocked with Cot1 DNA. The lack of hybridization under these conditions to the arms of endogenous human chromosomes suggests that the HAC did not acquire DNA sequences by telomere fragmentation. The chromosomes were counterstained with 4′,6-diamidino-2-phenylindole.