Table 2.
Mitotic stability of the HAC in clonal cell lines 64b5 and 69a12
| Clone | Days | G418 | No. of HAC(s)
|
Integration, % + HAC | |||
|---|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | ||||
| 64b5 | 0 | + | 0 | 92 | 4 | 4 | 0 |
| 64b5 | 18 | + | 0 | 100 | 0 | 0 | 0 |
| 64b5 | 36 | + | 0 | 87 | 9 | 0 | 4 (4) |
| 64b5 | 108 | + | 0 | 89 | 3 | 0 | 8 (1) |
| 64b5 | 18 | − | 4 | 88 | 4 | 4 | 0 |
| 64b5 | 36 | − | 4 | 92 | 4 | 0 | 0 |
| 64b5 | 72 | − | 0 | 88 | 4 | 0 | 8 (2) |
| 64b5 | 108 | − | 0 | 70 | 6 | 4 | 20 (9) |
| 69a12 | 0 | + | 13 | 54 | 33 | 0 | 0 |
| 69a12 | 81 | − | 9 | 56 | 0 | 9 | 26 (9) |
Cell lines 64b5 and 69a12 were passaged 1:8 every 3 days, in the presence (+) or absence (−) of G418, for the indicated number of days. Metaphase spreads were analyzed for the presence of the HAC with probe L2H2UN1. Shown is the percentage of metaphase spreads containing 0, 1, 2, or 3 HACs and the percentage of metaphase spreads displaying an integration event. In parentheses, the percentage of metaphase spreads displaying both an integration event and an extrachromosomal element simultaneously is shown.