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In vitro stimulation of proliferation of splenic B cells from wild-type and F/A-2+/+ mice. Splenic lymphocytes (105 cells per well) of 4-week-old mice were stimulated with LPS, goat F(ab′)2 anti-mouse μ chain, anti-CD40 antibody, or IL-4, either alone or in combination for 2 days (upper graph) or 3 days (lower graph). Cells of wild-type and F/A-2+/+ spleens displayed similar proliferative responses. Data are presented as mean of triplicate cultures.
