Expression of glnA in RB9132 (ΔntrB) carrying NtrB derivatives containing the transmitter module. (A) Cells grown in GLBgln carried the following plasmids. Column 1, pgln62 (NtrB); column 2, pTrc99B (vector); column 3, pJH391 (vector); column 4, pB1 (NtrB); column 5, pFB1 (λN-NtrB); column 6, pBHNG1 (NtrBHNG); column 7, pFBHNG1 (λN-NtrBHNG); column 8, pgln62–287 (NtrBD287G); and column 9, pFBHNG287 (λN-NtrBHNG287). Cells containing pFB1, pFBHNG1 and pFBHNG287 (columns 5, 7 and 9) were grown in the presence of 10 μM IPTG, cells containing pBHNG1 (column 6) in the presence of 50 μM IPTG. (B) Columns as in A, but cells were grown in Ggln medium.