Skip to main content
. 1999 Jan 19;96(2):604–609. doi: 10.1073/pnas.96.2.604

Figure 5.

Figure 5

Expression of glnA in RB9132 (ΔntrB) carrying different NtrB derivatives lacking the G domain. Cells were grown in Ggln or GLBgln as indicated. (A) Analysis of NtrB-derivatives fused to the N-terminal domain of λ repressor. Cells contained the following plasmids. Columns 1 and 8, pgln62 (NtrB); columns 2 and 9, pJH391 (vector); columns 3 and 10, pFB1 (λN-NtrB); columns 4 and 11, pFBSHN1 (λN-NtrBSHN); columns 5 and 12, pFBHN1 (λN-NtrBHN); column 6, pFBH1 (λN-NtrBH); column 7, pFBSH1 (λN-NtrBSH). (B) Analysis of NtrB derivatives cloned under the control of the trc promoter. Cells contained the following plasmids. Columns 1 and 5, pTrc99B (vector); columns 2 and 6, pB1 (NtrB); columns 3 and 7, pBSHN1 (NtrBSHN); columns 4 and 8, pBHN1 (NtrBHN). All cultures were grown in the presence of 10 μM IPTG with the exception of cells carrying pgln62 (no IPTG), cells carrying pFBSH1 (50 μM IPTG), and cells carrying pFBH1 (100 μM IPTG).