Table 2.

Yield of AP-RDA markers (BUF–ACI)

Primer name	No. of clones						No. of clones isolated in both C1 and C2	No. of independent markers
	Total		Independent (% total clones)		Polymorphic (% independent clones)
	C1	C2	C1	C2	C1	C2
Single primer
AP-3	34	69	22 (65)	22 (32)	5 (23)	7 (32)	4*†	8
AP-5	40	43	23 (58)	5 (12)	5* (22)	4† (78)	3	4
AP-6	87	79	34 (39)	26 (33)	5 (15)	6‡ (23)	2	9
AP-7	83	79	46 (55)	16 (20)	6 (13)	5§ (32)	3	8
Subtotal	241	270	125 (51)	69 (26)	21 (17)	22 (32)	12	29
AP-9		39		15 (38)		4 (27)		4
AP-11		66		21 (32)		1 (5)		1
AP-12		73		37 (51)		6 (16)		6
Subtotal		178		73 (41)		11 (15)		11
Combination
AP-3 + AP-6		45		14 (31)		6‡ (43)		3
AP-7 + AP-8		107		17 (16)		9§ (53)		4
Subtotal		152		31 (20)		15 (48)		7
Total	241	600	125 (51)	173 (29)	21 (17)	48 (28)	12	47

^*One clone repeatedly was isolated from C1 and C2 of AP-3 amplicon and C1 of AP-5 amplicon. 

^†Two clones repeatedly were isolated from C1 and C2 of AP-3 amplicon and C2 of AP-5 amplicon. 

^‡Three clones repeatedly were isolated from C2 of AP-6 amplicon and C2 of amplicon with AP-3 and AP-6. 

^§Five clones repeatedly were isolated from C2 of AP-7 amplicon and C2 of amplicon with AP-7 and AP-8.