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. 2003 Feb 1;111(3):333–340. doi: 10.1172/JCI15555

Figure 2.

Figure 2

Determination of FK mRNA in primary vascular tissues. Mice lacking apoE were maintained on the Western diet for 15 weeks. Thoracic and abdominal aortas were opened longitudinally, and the luminal endothelial cells (n = 11 mice), aortic media (n = 8 mice), and the aortic root (n = 14 mice) were isolated as described in Methods. Real-time PCR was performed to quantify FK mRNA expression, and the results are expressed as the mRNA FK/β-GUS mRNA. Each data point represents the mean of duplicate measurements.