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CpG content upstream of GPC3 and SYBL1 and determination of the transcription-initiation sites for SYBL1. Percentages of GC and CpG were calculated by using a 100-bp sliding window across the GPC3 (A) and SYBL1 (B) promoter regions. Transcription-initiation sites for the two genes are indicated with arrowheads. (C) Determination of the transcription-initiation sites for SYBL1 by primer extension. The initiation site for GPC3 has been determined (13).
