Figure 4.

In vitro methylation analysis of the GPC3 and SYBL1 promoters. pCAT-Control, containing the SV40 promoter/enhancer driving the CAT cDNA (open bars), pCAT-GPC3, containing the GPC3 minimal promoter driving the CAT cDNA (hatched bars), or pCAT-SYBL1, containing the SYBL1 minimal promoter driving the CAT cDNA (shaded bars), was methylated in vitro as indicated with HhaI methylase, HpaII methylase, or SssI methylase and transfected transiently into HeLa cells. Resultant CAT activity was normalized to β-galactosidase activity from a cotransfected SV40-βGAL (pSV-βGAL) construct and plotted along the y axis.