Figure 5.

Activation of SYBL1 expression after 5aCdr treatment of Y and inactive X hybrids. SYBL1 expression and MIC2 expression were examined in hamster–human somatic-cell hybrids by using RT-PCR (as described in Materials and Methods). PCR products were derived from reverse transcriptase reactions that contained 0.5 μg of RNA (0.5), 1.0 μg of RNA (1.0), or 1.0 μg of RNA without reverse transcriptase (−RT). Cell lines and 5aCdr-treated cultures analyzed included GM06317, an untreated hybrid with a Y chromosome, mass cultures of GM06317 isolated at different times after 5aCdr treatment, THX88, an inactive X hybrid (Xi), a mass culture of THX88 after 5aCdr treatment, and GM06318, an active X hybrid (Xa).