Figure 6.

Expression and DNA methylation analysis of GPC3 in peripheral blood leukocytes. (A) RNA from Caco-2 cells (lane 1), human male peripheral blood leukocytes (lane 2), and human female peripheral blood leukocytes (lane 3) was reverse transcribed and amplified in multiplex by using both GPC3- and actin gene-specific primers. A control PCR with GPC3 and actin cDNA preparations was electrophoresed in lane 4 for comparison. (B) Southern blotting of the GPC3 promoter was carried out as in Fig. 3 by using human male peripheral blood leukocyte DNA (lanes 1–3) or female peripheral blood leukocyte DNA (lanes 4–6).