Figure 2.

B cell lymphoma LEC uses CD86 molecules to costimulate expression of Nur77 but not Egr-1 protein. 2B4 thymocytes were cocultured with LEC and different concentrations of p17 (5, 1 or 0 μg/ml). LEC had been preincubated with 5 μg/ml anti-CD86, 10 μg/ml anti-K^k, or no antibody. After 2 hr of coincubation, thymocytes were separated from LEC and whole-cell lysates were analyzed by Western blot analysis using antibodies to Nur77 (Upper) or to Egr-1 (Lower). Similar results were obtained after 1 and 4 hr of coincubation (data not shown).