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. 2003 Jan 15;111(2):197–208. doi: 10.1172/JCI16010

Figure 8.

Figure 8

Cytotoxicity of GD3 in mouse hepatocytes. ASMase+/+ (a) and ASM–/– (b) hepatocytes were treated with GD3 (10 μM) with or without HP preincubation, and cell death was determined by Hoechst 33258 and propidium iodide staining to quantify apoptosis and necrosis after 8 hours. Caspase 3 activity was determined in cell extracts from the fluorescence of AMC fragments. Results are expressed as means ± SD (n = 4 independent experiments). *P < 0.05 versus control, and ** P < 0.05 versus GD3 without HP preincubation.