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Overlay of TOF-SIMS mass-resolved images of negatively charged secondary ions of the system DPPC/d62DPPG/SP-B (80:20:0.4 mol %) after LB-transfer at 30 mN/m (left) and at 50 mN/m (middle) as well as after LS-transfer at 50 mN/m (right). The fragment ions with the mass/charge ratio of 2 (D−, d62DPPG-specific), 42 (CNO−, protein-specific), and 255 (palmitidic acid anion, DPPC-specific) are shown (rows 1–3) and used to perform an overlay (row 4).
