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EGFP fluorescence spectrum under 488 nm excitation at different temperatures. Solid lines show an approximation of the spectrum by two Lorentzians centered at 510 nm and at 540 nm. A temperature rise from 25°C to 34°C results in the decrease in the peak intensity, ΔI = 7.3% and in a red-shift of Δλ = 0.8 nm. The intensity of the 510 nm peak at 25°C is adopted as 100%.
