Figure 2.

Northern blot analysis of representative tissues confirming the complete and selective inactivation of the GPI-DAF gene. (A) (a) GPI-DAF mRNAs were expressed in the wild-type (+/+) but not the knockout (−/−) mouse intestine (In) or lung (Lu). Two alternatively spliced GPI-DAF mRNAs were detectible; (b) the membrane was stripped and rehybridized with a control cDNA (glyceraldehyde-3-phosphate dehydrogenase; GAPDH) probe to show equivalent RNA loading. (B) (a) GPI-DAF was expressed in wild-type but not in knockout mouse testis; (b) TM-DAF gene was expressed in both the wild-type and the GPI-DAF knockout mouse testis; (c) rehybridization of the membrane with a GAPDH probe. Positions of the 18S and 28S ribosomal RNAs are marked on the left.