Figure 1. Testing of T cell lines (TCL) isolated from biopsy specimens of a DQ2⁺ DQ8⁺ patient (CD465) stimulated in parallel with (A) chymotrypsin-treated gluten, (B) chymotrypsin-treated recombinant AJ133612 α-gliadin, and (C) overlapping AJ133612 α-gliadin peptides against the whole panel of TG2-treated AJ133612 α-gliadin peptides (1409–1457).

Peptides were tested at 10 μM, and CD114 (DQA1*0501/DQB1*0201) B lymphoblastoid cells were used as APCs. TG2-treated gluten and TG2-treated recombinant AJ133612 α-gliadin were used as positive controls. Responses are given as the stimulation index. α-GPs, α-gliadin peptides; r–α-gli, recombinant α-gliadin.