Figure 2. DEX-treated BMMs differentiate into osteoclasts but fail to spread.

(A) WT and GR^oc–/– BMMs were cultured with M-CSF and RANKL with or without DEX (100 nM). At day 0 and day 5, RNA was extracted, and the expression of osteoclastogenic markers was analyzed by RT-PCR. GAPDH served as loading control. (B) WT and GR^oc–/– BMMs were cultured with M-CSF and RANKL with or without increasing concentrations of DEX. Five-day osteoclastogenic cultures were stained for TRAP activity. Magnification, ×250. (C) Statistical analysis of the number of WT and GR^oc–/– spread TRAP-positive multinucleated cells/well. *P < 0.001, **P < 0.0001 versus DEX-untreated WT.