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. 2006 Jul 24;103(31):11485–11490. doi: 10.1073/pnas.0601828103

Fig. 1.

Fig. 1.

BLM is phosphorylated during mitosis and upon SAC activation in a MPS1-dependent manner. (A) BLM and MPS1 coimmunoprecipitate from HeLa nuclear extract. Immunoprecipitations with goat and rabbit preimmune sera serve as negative controls. (B) Hyperphosphorylation of BLM is diminished in MPS1-knockdown cells and is restored upon expression of siRNA-resistant RFP-MPS1. Knockdown of Vimentin (siVim) serves as a negative control. The arrows indicate hypo- and hyperphosphorylated BLM. (C) MPS1-dependent BLM phosphorylation at S144 and S1290/S1296. HeLa cells transfected with siMPS1 or siVim were treated with nocodazole and cell lysates were Western blotted with the indicated phospho-specific antibodies.