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TC-tagged proteins display mitochondrial localization. HeLa-cyt.c-GFP cells were stably (Smac, Omi, and AK2) or transiently (AIF) transfected with the vector encoding the corresponding TC-tagged protein and stained with the red dye ReAsH. (Scale bars: 20 μm.) Arrow indicates a cell transfected with AIF-TC; other cells in the field did not express the protein. Full-resolution images are shown in Fig. 5.
