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. 1999 Jan 19;96(2):640–645. doi: 10.1073/pnas.96.2.640

Figure 2.

Figure 2

pComBov expression cassette. Modified from pComb3H phagemid (19) on a pBluescript background (23). The CL was cloned from bovine genomic DNA. Digestion with SpeI and NheI removes the geneIII sequence, allowing expression of soluble Fab. Arrows indicate the position of cleavage of secretory sequences (pelB or ompA) during periplasmic expression of Fab. Amino acid resides (single-letter code) immediately after the cleavage site are the optimized bovine amino-terminal Ig sequences. RBS, ribosome binding site.